FIGURE 3.
Effect of siRNA knockdown of m1A-methylase subunits, TRM6 and TRM61. (A) Western blot showing efficiency of siRNA treatment of MDA-MB231 cells. The siRNA treatment causes the decrease in protein level after 3 d of treatment. GAPDH level is used as a control. (B) Growth of control versus TRM6 and TRM61 siRNA-treated cells. (C) Representative array image from control and siTRM6-treated cells. (D) Heat map of hypomodification in control versus TRM6 and TRM61 knockdown cells. Purple arrows show tRNA species with significant increase in hypomodification in the siRNA-treated cells. Blue arrow indicates initiator-tRNAMet, which, as expected, is not hypomodified at all times. (E) Heat map of relative tRNA abundance of the control and siRNA-treated cells shows no change in tRNA abundance in all cases.