FIGURE 6.

Analysis of 9S rRNA processing. Equivalent amounts (5 μg) of total steady-state RNA extracted from cultures that either had been grown at 30°C or had been shifted to 44°C for 120 min, as described in Materials and Methods, were separated in a 6% polyacrylamide/7 M urea gel and electroblotted onto a Biotrans⁺ membrane. The membrane was probed with ³²P-end–labeled oligonucleotide complementary to the mature 5S rRNA (PB5S; Babitzke and Kushner 1991). The left arrows indicate the mature 5S rRNA. (A) rne and rng alleles present in six-to-eight copies/cell. SK9714 (rne⁺), SK9937 (rne-1), SK9987 (rneΔ645), SK3541 (rng-248), and SK3543 (rng-219). (B) rne and rng alleles present in single copy/cell. SK10143 (rne⁺), SK10144 (rne-1), SK2685 (rneΔ645), SK3563 (rng-248), and SK3564 (rng-219). PF denotes the processed fraction, which is defined as the amount of mature 5S rRNA divided by the total amount of the 5S rRNA (processed and unprocessed).