FIGURE 7.

Analysis of tRNA maturation in rneΔ1018/rng-219 and rneΔ1018/rng-248 mutants when the rng alleles were present in single copy. Steady-state RNA (5 μg/lane) isolated from cultures grown either at 30°C or after shift to 44°C was analyzed as described in Materials and Methods. PF denotes the processed fraction, which is defined as the amount of a given mature tRNA divided by the total amount of tRNA transcribed. The numbers represent the average of three to four independent determinations. The standard deviations from the mean are presented in Table 4. Arrows in the right margins indicate the position of each mature tRNA. RNA levels were normalized as described in Materials and Methods.