Table 1.

Phenotypes of strains expressing TolCΔ2–22

Strain	Plasmid	Suppressor mutation* (alteration)	Zone of inhibition†			Sensitivity‡
			SDS	DOC	NB	TLS	ColE1
RAM1330	pBAD24	wt	26	16	16	R	R
RAM1330	pBAD24-TolC	wt	6	6	10	S	S
RAM1330	pBAD24-TolCΔ2–22	wt	25	14	16	R	R
S1	pBAD24-TolCΔ2–22	secY/prlA9 (D69G)	19	8	13	S	S
S7	pBAD24-TolCΔ2–22	secY (R74C)	21	13	16	S	S
S14	pBAD24-TolCΔ2–22	Unknown	21	14	17	S	S
S15	pBAD24-TolCΔ2–22	Unknown	22	14	17	S	S
S24	pBAD24-TolCΔ2–22	secY/prlA666 (S67F)	23	11	14	S	S
S27	pBAD24-TolCΔ2–22-Bla42-TolCΔ2–22	wt	6	6	10	S	S
S29	pBAD24-TolCΔ2–22	Unknown	26	14	17	S	S
S31	pBAD24-TolCΔ2–22-Bla42-TolCΔ2–22	wt	6	6	10	S	S

*All strains are derived from RAM1330 (MC4100 Δara ΔtolC : : Km^r); wt, wild-type allele; prlA, suppressor mutations in secY.

†Growth inhibition diameters are in mm. The diameter of the disks was 6 mm. SDS (10 μl of a 10 % solution); DOC, sodium deoxycholate (10 μl of a 10 % solution); NB, novobiocin (30 μg).

‡Sensitivities to phage TLS and ColE1 were determined by spotting dilutions at 10⁻⁴.