Table 1.
Strains used in this study
Cb, Gm, Km, Sm, Su, Tc and Hg, resistance to antibiotics carbenicillin, gentamicin, kanamycin, streptomycin, sulphonamide, tetracycline and to mercury ions, respectively; Uv, ultraviolet light protection; Ant, Cap, Nah, Phn, Sal, Tol and Xyl, ability to degrade anthracene, ε-caprolactam, naphthalene, phenanthrene, salicylic acid, toluene and (m- and p-) xylenes, respectively.
| Strain | Plasmid | Size (k)b | Plasmid phenotype | Source of isolate | Reference or provider* |
|---|---|---|---|---|---|
| P. putida PpG7 | NAH7 | 83 | Nah Sal | Coal-tar-contaminated soil, CA, USA | Dunn & Gunsalus (1973) |
| P. putida BS 202 | NPL-1 | 100 | Nah Sal | Coal-tar-contaminated soil near coal tar mine, Makeevka, Ukraine | Izmalkova et al. (2006); RAS |
| Pseudomonas spp. 8C | p8C | 110 | Nah Sal Phn | Oil-contaminated soil, Tumen region, Western Siberia, Russia | Izmalkova et al. (2006); RAS |
| Pseudomonas spp. 15C | p15C | 110 | Nah Sal Phn | Oil-contaminated soil, Tumen region, Western Siberia, Russia | Izmalkova et al. (2006); RAS |
| P. putida BS 238 | pBS2 | 130 | Nah Sal | Soil from territory of metallurgical plant, Nizhniy Tagil, Russia | Izmalkova et al. (2006); RAS |
| P. putida BS 3710 (cys−) | pBS216 | 83 | Nah Sal Phn | Soil from territory of metallurgical plant, Magnitogorsk, Russia | Izmalkova et al. (2006); RAS |
| P. putida BS 639 (cys−) | pBS240 | 160 | Nah | Coke chemical plant, Kemerovo, Russia | RAS |
| P. putida BS 638 (cys−) | pBS243 | 160 | Nah | Soil from territory of metallurgical plant, Magnitogorsk, Russia | RAS |
| P. putida BS 394 (cys−) | pBS265 | 130 | Cap | Chemical plant sewage, Severodonetsk, Ukraine | Krasowiak et al. (2002); RAS |
| P. putida BS 394 (cys−) | pBS267 | 130 | Cap | Chemical plant sewage, Severodonetsk, Ukraine | Krasowiak et al. (2002); RAS |
| P. putida BS 394 (cys−) | pBS268 | 85 | Cap | Chemical plant sewage, Kemerovo, Russia | Mavrodi et al. (2003); RAS |
| P. putida BS 3701 | pBS1141, pBS1142 | 100, 60 | Nah Sal Phn Ant cryptic | Coke chemical plant, Vidnoe, Moscow region, Russia | Izmalkova et al. (2006); RAS |
| P. putida BS 3750 | pBS1181 | 120 | Nah Sal Phn | Oil-contaminated soil, Tumen region, Western Siberia, Russia | Izmalkova et al. (2006); RAS |
| P. putida BS 3790 | pBS1191, pBS1192 | 100, 60 | Nah Sal Phn Ant cryptic | Oil-contaminated soil, Tumen region, Western Siberia, Russia | Izmalkova et al. (2006); RAS |
| P. putida NCIB 9816-4 | pDTG1 | 83 | Nah | Coal-tar-contaminated site, Bangor, Wales, UK | Dennis & Zylstra (2004); Evans et al. (1965) |
| Unknown† | pFKY1 | 200 | Nah Sal | Oil-contaminated site, Japan | M. Tsuda, unpublished data |
| P. putida M | pM3 | 75 | Sm Tc Uv | Sewage and soil from different industrial and agricultural locations in Belarus and Azerbaijan | Greated et al. (2000); Titok et al. (1991) |
| P. putida M (pro−) | pM77 | 75 | Sm Tc | Soil from the area of sewage treatment plant, Minsk, Belarus | Krasowiak et al. (2002); BSU |
| P. putida M (pro−) | pM80 | 75 | Sm Tc | Soil from the area of sewage treatment plant, Minsk, Belarus | Krasowiak et al. (2002); BSU |
| P. putida AC34 | pMG18 | 100 | Cb Gm Km Sm Su Hg | Japan | Jacoby & Matthew (1979) |
| P. putida 10a | pNL4 | 75 | Nah Sal | Soil from a distillery area, Minsk, Belarus | Leuchuk et al. (2006); BSU |
| E. coli C600‡ | pNL15‡ | 75 | Sm | Soil from a petrol station area, Minsk, Belarus | Leuchuk et al. (2006); BSU |
| Pseudomonas fluorescens 41a | pNL22 | 100 | Nah Sal | Soil from a petrol station area, Minsk, Belarus | Leuchuk et al. (2006); BSU |
| P. putida 21a | pNL25 | 75 | Nah Sal | Soil from a railway station area, Minsk, Belarus | Leuchuk et al. (2006); BSU |
| Pseudomonas spp. 58 | pNL29 | nd§ | Nah Sal | Soil from a petrol station area, Minsk, Belarus | Leuchuk et al. (2006); BSU |
| Pseudomonas aeruginosa 56 | pNL31 | nd§ | Nah Sal | Soil from the roadside, Minsk, Belarus | Leuchuk et al. (2006); BSU |
| P. fluorescens 18d | pNL60 | 120 | Nah Sal | Soil from the foundry area, Homel, Belarus | Leuchuk et al. (2006); BSU |
| Pseudomonas aureofaciens OV17 | pOV17 | 85 | Nah | Oat rhizosphere from oil-contaminated soil, Western Siberia, Russia | RAS |
| P. putida SN11 | pSN11 | 83 | Nah Sal | Salt-contaminated soil from chemical plant, Berezniki, Ural, Russia | Izmalkova et al. (2006); RAS |
| P. putida SVS15 | pSVS15 | 90 | Tol Xyl | Piece of rubber from used-car-tyre storage, Minsk, Belarus | Sentchilo et al. (2000) |
| P. putida mt-2 (PaW1) | pWW0 | 116.58 | Tol Xyl | USA | Greated et al. (2002); Williams & Murray (1974) |
| P. aeruginosa ML 4262 | R2 | 73 | Cb Sm Su Uv | Japan | Kawakami et al. (1972) |
*RAS and BSU, bacterial strain collections obtained from Russian Academy of Sciences and Belarus State University, respectively.
†Plasmid obtained by exogenous isolation.
‡Plasmid was labelled with mini-Tn5 (Km) and transferred by conjugation into E. coli C600 (selection for Km Nah− phenotype) from the natural host P. fluorescens 42 (Leuchuk et al., 2006).
§Not determined.