Table 3.
Analysis of possible insertions in two sites of the IncP-9 backbone by PCR and hybridization with plasmids representing the eight best-characterized subgroups
| Subgroup | Plasmid phenotype* | Plasmid | ruvB/ruvB1–ruvA | orf39A/607–orf175/613 | ||
|---|---|---|---|---|---|---|
| Hybridization signal | Presence of insertion† | Hybridization signal | Presence of insertion† | |||
| α | R | pM3 | + | − | − | + |
| β | Tol | pWW0 | + | (−) | − | (+) |
| Nah | NPL-1, pBS2, pBS1141, p8C, p15C, pBS1181 | + | − | + | − | |
| Nah | pBS1191 | + | − | − | + | |
| γ | Cap | pBS265, pBS267 | + | − | − | + |
| δ | Nah | pBS216, pSN11 | − | (+) | + | (−) |
| ε | R | R2 | + | − | − | + |
| R | pMG18 | − | + | − | + | |
| θ | Tol | pSVS15 | + | − | + | − |
| ŋ | R | pNL15 | + | − | − | + |
| ζ | Nah | pNL60 | + | (−) | + | (−) |
*Tol, Nah and Cap, degradation of toluene/xylenes, naphthalene and caprolactam, respectively; R, antibiotic resistance.
†+ or − indicates predicted presence or absence of insertions in uncharacterized plasmids. (+) or (−) indicates known presence or absence of insertions in characterized plasmids (pWW0, δ and ζ plasmids).