Fig. 3.

FCV p32, p39 and p30 proteins behave as integral membrane proteins. (a) Western blot analysis of soluble (Sol) or membrane (Mem) fractions from cells transfected with ECFP or ECFP p32, p39 and p30 fusion proteins. (b) Western blot analysis of membrane preparations subsequently extracted with either PBS, Na₂CO₃, EDTA, NaCl or SDS to examine the mechanism of association of the FCV p32, p39 and p30 proteins. Membranes from transfected cells were prepared as in (a), extracted with the various reagents and collected by high-speed centrifugation. Membrane-associated and solubilized proteins were then analysed by Western blot analysis using either anti-EGFP, anti-ERp57 or anti-Calnexin antisera.