Fig. 5.
FCV p30 and p39 proteins affect the accumulation of a transiently expressed HRP protein containing the KDEL ER retention signal, as does FCV replication. (a) Cells expressing HRPKDEL were colorimetrically assayed 24 h post-transfection using ELISA substrate. Absorbance was measured at 450 nm. The average signal observed during co-expression of the FCV p32, p39, p30 and p76 m proteins as well as during FCV replication following viral RNA transfection is presented as a percentage of the HRPKDEL/blank plasmid control. Error bars indicate sem from experiments and colorimetric assays performed in triplicate. (b) Western blot of the same lysates performed using anti-myc antibody to detect the HRPKDEL and anti-GAPDH.