Table 4.
Filtering treatment | k | Reads after filtering (no.) | Contig size (kb) | Contigs (no.) | Velvet parameter (value)* | |||||
---|---|---|---|---|---|---|---|---|---|---|
Median | Maximum | Total | All | >1 kb | >5 kb | Exp_cov | Cov_cutoff | |||
Unfiltered | 21 | 5079235 | 8 | 25 | 246 | 237 | 36 | 16 | 290 | 42 |
25 | 5079235 | 14 | 44 | 246 | 178 | 30 | 13 | 250 | 41 | |
Singletons removed | 21 | 1682785 | 13 | 32 | 231 | 65 | 27 | 14 | 210 | 82 |
Overall quality: | ||||||||||
>1900 | 21 | 2338321 | 3 | 16 | 292 | 765 | 64 | 16 | 110 | 11 |
>1700 | 21 | 4156964 | 7 | 32 | 264 | 383 | 46 | 13 | 220 | 26 |
25 | 4156964 | 11 | 32 | 243 | 150 | 32 | 16 | 190 | 44 | |
Quality truncation, length >29 | 21 | 2609325 | 7 | 15 | 242 | 233 | 36 | 13 | 65 | 26 |
Truncated to length: | ||||||||||
40 | 21 | 5079235 | 14 | 45 | 251 | 256 | 21 | 12 | 190 | 25 |
25 | 5079235 | 18 | 44 | 242 | 114 | 23 | 12 | 160 | 38 | |
35 | 21 | 5079235 | 29 | 65 | 246 | 176 | 18 | 8 | 150 | 26 |
25 | 5079235 | 31 | 68 | 242 | 99 | 13 | 10 | 110 | 30 | |
30 | 21 | 5079235 | 24 | 68 | 240 | 90 | 20 | 11 | 105 | 32 |
25 | 5079235 | 54 | 68 | 237 | 61 | 8 | 7 | 60 | 24 |
*Each dataset was assembled using Velvet-0.7.31 (see Methods). Exp_cov, Optimal expected coverage; cov_cutoff, optimal coverage cut-off.