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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Biochem Pharmacol. 2009 Dec 11;79(11):1553–1561. doi: 10.1016/j.bcp.2009.12.004

Fig. 2.

Fig. 2

Clonogenic experiments using CHO/AA8, U251 and EMT6 cell lines treated with various agents under conditions of oxygenation or oxygen deficiency. CHO/AA8 (oxygenated, panel A; oxygen-deficient, panel B), U251 cells (oxygenated, panel C; oxygen-deficient, panel D), and EMT6 cells (oxygenated, panel E; oxygen-deficient, panel F) were treated with varying concentrations of KS119 (▲), 90CE (■), or Onrigin (●). The vertical axis indicates the percent survival over untreated controls; the horizontal axis indicates the concentration of the agent employed in μM. Oxygen depleted samples were treated for 24 h in the presence of 2 U/ml of glucose oxidase, 120 U/ml of catalase and 25 mM glucose, before harvesting and plating of cells to measure cloning efficiency. All points are the result of at least three independent experimental determinations ±SEM.