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. Author manuscript; available in PMC: 2011 May 17.

Published in final edited form as: Chem Res Toxicol. 2010 May 17;23(5):918–925. doi: 10.1021/tx100003w

(i) Reaction of BQ with NBT in 50% acetonitrile in a pH 7.4 phosphate buffer at 25 °C. Absorbance readings were performed at 412 nm. Using [NBT]₀ = 0.1 mM. BQ concentrations were varied from (a) 0.0 mM (b) 0.0125 mM (c) 0.025 mM (d) 0.05 mM (e) 0.1 mM (f) 0.2 mM (g) 0.4 mM (h) 0.8 mM. (ii) NBB was reacted with 0.1mM NBT in a phosphate buffered 50% acetonitrile. Initial NBB concentrations were (a) 0.0 mM (b) 0.1 mM (c) 0.25 mM (d) 0.5 mM (e) 1 mM (f) 2 mM (g) 4 mM. (iii) NBT at a concentration of 0.1 mM was reacted with {(a) 0 (b) 0.01 (c) 0.02 (d) 0.05} mM TDI in acetone.

(i) Reaction of BQ with NBT in 50% acetonitrile in a pH 7.4 phosphate buffer at 25 °C. Absorbance readings were performed at 412 nm. Using [NBT]₀ = 0.1 mM. BQ concentrations were varied from (a) 0.0 mM (b) 0.0125 mM (c) 0.025 mM (d) 0.05 mM (e) 0.1 mM (f) 0.2 mM (g) 0.4 mM (h) 0.8 mM. (ii) NBB was reacted with 0.1mM NBT in a phosphate buffered 50% acetonitrile. Initial NBB concentrations were (a) 0.0 mM (b) 0.1 mM (c) 0.25 mM (d) 0.5 mM (e) 1 mM (f) 2 mM (g) 4 mM. (iii) NBT at a concentration of 0.1 mM was reacted with {(a) 0 (b) 0.01 (c) 0.02 (d) 0.05} mM TDI in acetone.