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. Author manuscript; available in PMC: 2011 Mar 5.
Published in final edited form as: Cell. 2010 Mar 5;140(5):678–691. doi: 10.1016/j.cell.2010.01.003

Figure 4. Enrichment of H3.3 at active and repressed genes is Hira-dependent.

Figure 4

A) Immunoblots showing expression of H3.3-EYFP in wild-type and Hira−/− ES cells, with antibodies as indicated.

B–E) Native ChIP-seq profiles of H3 variants, in wild-type ES cells (B-D) and Hira −/− ES cells (E) across the TSS and TES for highly active (red), medium expressing (black), or low expressing (green) CpG rich genes, with data represented as in Figure 2. See also Figure S4.

F) H3.3 enrichment at the TSS of repressed bivalent gene Pparg is Hira-dependent.

G) H3.3 enrichment at the active, ribosomal-protein coding gene Rps19 is Hira-dependent.