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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Cell Physiol. 2010 Jul;224(1):210–217. doi: 10.1002/jcp.22116

Fig. 1. β1 integrins promote IGF-IR activation and increase GLI1 expression.

Fig. 1

A: PC3-β1-shRNA or PC3-cont-shRNA transfectants were serum starved and stimulated with or without IGF-1. Cell lysates were immunoprecipitated using Abs to IGF-IRβ and immunoblotted using Abs to phosphotyrosine (p-Tyr, PY20) or IGF-IRβ (left panels). In some experiments, cell lysates from IGF-1 treated PC3-β1-shRNA or PC3-cont-shRNA transfectants were immunoblotted using Abs to p-AKT or AKT (right panels). B: PC3-vector, PC3-β1-shRNA or PC3-cont-shRNA were detached, lysed and immunoblotted using Abs to GLI1, β1 (C-18) or FAK, as a loading control. C: PC3 parental, PC3-vector, PC3-cont-shRNA or PC3-β1-shRNA cells were transfected with pCMV-β-galactosidase and 8×3'GLI-BS pδ51LucII reporter construct. GLI reporter activity was analyzed by luciferase assay. The data were normalized using β-galactosidase activity. Data are expressed as means ±SEM (*p=0.0072).