FIGURE 1.

Cigarette smoke exposure decreases DC numbers in the lung. A, Lung cells were isolated from smoke-exposed and non-smoke-exposed control mice and flow cytometric analysis was used to identify DCs (CD11c⁺/AF^low). Macrophages (MAC) were identified as (CD11c⁺/AF^high). B, Absolute numbers of lung DCs from A. Data represent means ± SEM; n = 8. Statistical analysis was performed using the Student t test; p < 0.05. C, CD11c⁺/AF^low lung DCs were isolated from smoke-exposed and control mice 15 h following OVA administration and cocultured with DO11.10 CD4⁺ T cells. IL-2 was measured in cell culture supernatants by ELISA.