Table I.
In vitro OVA-specific cytokine productiona
| Control | Smoke | |
|---|---|---|
| IL-5 | ||
| Medium | 23.3 ± 17.5 | 39.7 ± 23.2 |
| OVA | 746.7 ± 69.8b | 549.1± 95.1b |
| IL-10 | ||
| Medium | 41.7 ± 16.0 | 51.0 ± 16.0 |
| OVA | 723.6 ± 96.9b | 643.9 ± 69.3b |
| IL-13 | ||
| Medium | 277.6 ± 147.4 | 392.7 ± 109.8 |
| OVA | 8880.7 ± 1058.5b | 9483.5 ± 1425.5b |
a
Smoke-exposed and control mice were injected i.v. with DO11.10T cells during the last week of exposure, followed 24 h later by intratracheal administration of 600μg of OVA. Four days later, TLN cells were isolated and placed in culture in the presence of either medium alone or OVA. Cytokine production was assessed in cell culture supernatants by ELISA. Data are expressed as means ± SEM (n = 9–11).Statistical analysis was performed using one-way ANOVA; p < 0.05.
b
Statistically significant compared to medium alone.