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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Alcohol Clin Exp Res. 2009 Aug 10;33(11):1945–1955. doi: 10.1111/j.1530-0277.2009.01032.x

Figure 4. Effect of Ethanol on Carbachol-Stimulated PKC Activity.

Figure 4

Hippocampal neurons were pretreated with 50 or 75 mM ethanol for 15 min; 100 μM carbachol was then added to some of the samples (solid bars), and cells were incubated for an additional 30 min. Cell lysates were collected and equal amounts of proteins were incubated with a fluorescent PKC substrate as described in Materials and Methods. At the end of the reaction the mixture was separated on an agarose gel and the bands corresponding to the phosphorylated peptide were quantified and normalized to controls. Results are the mean (± SEM) of three independent experiments. (*p<0.05 compared to control; #p<0.05, compared to carbachol alone).