Figure 5.

LC3_B and GATE-16 overexpression differently affects phagophore appearance. (A) Control HeLa cells and HeLa cells stably expressing GFP-LC3_B or GFP-GATE-16 were starved for 2 h in EBSS medium fixed and immunostained with anti-Atg16 antibodies. Quantification of Atg16-labelled puncta structures from three independent experiments is presented at the right panel. Arrowheads represent Atg-16-labelled puncta. Scale bar: 20 μm. (B) HeLa cells stably expressing GFP-LC3_B (left panel) or GFP-GATE-16 (middle panel) were starved for 2 h in EBSS medium. The cells were fixed and their cryo sections were immunolabelled as in (Figure 4B). Quantification of the size of GFP-labelled structures (right panel) from three independent experiments is presented. ^*P<0.05, ^**P<0.001.