Fig 4.
Prostaspheres form acinar structures in immunocompromised mice. Whole prostaspheres were isolated, mixed with Matrigel and injected subcutaneously into mice either with or without UGSM. Glandular-like structures were recovered 8–12 weeks after subcutaneous implantation of whole prostaspheres (containing from 5 × 104 to 1 × 106 cells) suspended in Matrigel. A: H&E staining of graft containing 2 × 105 rat UGSM only, graft containing regenerated prostate tissue induced by prostaspheres without UGSM, and graft of regenerated prostate tissue formed by combining whole prostaspheres with 2 × 105 rat UGSM. B: IHC analysis of the expression of CK5, p63, PSCA, AR,CK8, and PSA in normal human prostate tissue sections and acini induced by prostaspheres (regenerated graft).