A. RT-PCR showing Pax6(+5a), Pax6(−5a), and δ-catenin mRNA expression in Y79 cells transiently transfected with Pax6(+5a), Pax6(−5a), or pcDNA3.1 as vector control. GAPDH is used as internal mRNA control. B. Western blots showing Pax6(+5a), Pax6(−5a), and δ-catenin protein expression in Y79 cells transiently transfected with Pax6(+5a), Pax6(−5a), or pcDNA3.1. Note: semi-quantitative measurements of the RT-PCR and Western blot are shown underneath each lane. Actin is used as protein loading control.