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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Exp Cell Res. 2010 Jan 11;316(6):1070–1081. doi: 10.1016/j.yexcr.2010.01.006

Fig. 5. Induced overexpression of Pax6(+5a) is accompanied by the reduction of Pax6(−5a).

Fig. 5

Immunofluorescent laser confocal light microscopy shows the time course of Pax6(+5a) and Pax6(−5a) expression in HeLa Tat-TetR-Pax6 cells double labeled using anti-Pax6(+5a) and anti-Pax6(−5a). A–C: Pax6(+5a) and Pax6(−5a) expression in HeLa Tat-TetR-Pax6 cells without (HeLa TR) induction of Pax6(+5a) overexpression. D–F: HeLa Tat-TetR-Pax6 cells at day 1 (HeLa tet d1) with induction of Pax6(+5a) overexpression. G–I: HeLa Tat-TetR-Pax6 cells at day 3 (HeLa tet d3) with induction of Pax6(+5a) overexpression. Note: compared with A, many cells in D show moderately increased Pax6(+5a) expression and show dramatically increased expression in G (arrows). On the other hand, Pax6(−5a) shows slightly decreased expression in cells in D but more pronounced decrease in expression in H when compared to A (arrowheads). A, D, and G: Pax6(+5a); B, E, and H: Pax6(−5a); C, F, and I: Merged Images. Bar: 25 µm.