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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Neurotoxicology. 2010 Apr 22;31(4):367–372. doi: 10.1016/j.neuro.2010.04.006

Figure 2.

Figure 2

(A) Beta-hydroxy toulene (BHT) and catalase, but not other antioxidants, attenuated rotenone’s UPS inhibitory effects. HEK cells were treated with 100 nM rotenone in combination with the various anti-oxidants for 24 h before being analyzed by flow cytometry. GFP-U is expressed as a percent of the increase in GFP-U caused by rotenone alone. A value of 100% would indicate no effect on rotenone, and 0% would indicate a complete reversal of rotenone’s effects (N=5–9). B: BHT and Trolox-C attenuated the formation of peroxides caused by 100 nM rotenone as measured by DCF flouresence (N=4–11 per data point). Note that although both reduced ROS produced by rotenone, only BHT attenuated UPS inhibiton.