Figure 4. Prior entrainment conditions determine the rate of damping in cells expressing KaiC^EE.

Cells were in LL at 30°C before and after the following entrainment conditions: (A) one 12 h dark pulse, (B) one 24 h dark pulse, (C) two 12 h dark pulses separated by one 12 h light pulse (i.e., 1.5 cycles of LD12:12), and (D) two 24 h dark pulses separated by one 24 h light pulse (i.e., 1.5 cycles of LD24:24). In all panels, the left ordinate is the luminescence level of the WT strain and the right ordinate is the luminescence level of the KaiC^EE strain. In panels A–D, the black traces are the WT luminescence and the red traces are the KaiC^EE luminescence (these traces are the average of duplicate measurements, see Figure S8 for all raw data). (E) Damping analysis as the number of days required for the amplitude of the rhythm to decrease to 1/e (≈36.79%) of the starting value. (F) Damping analysis as the number of cycles required for the amplitude of the rhythm to decrease to 1/e. In panels E and F, n = 5 for KaiC^WT and n = 7 for each of the KaiC^EE sample sets; error bars are SEM.