Skip to main content
. 2010 Jun 15;8(6):e1000396. doi: 10.1371/journal.pbio.1000396

Figure 5. hid downregulation by miR-263a is required for IOB formation.

Figure 5

(A) SEM of an adult eye expressing the endogenous hid gene from the EP line P[XP]d10274 under miR-263b-Gal4 control. (B) Normalized hid mRNA levels measured by qRT-PCR. RNA was extracted from pupal eye discs of the indicated genotypes at 30 h APF (before the IOB cells are lost in the mutant). Error bars represent mean ± SD of three independent biological replicates for each genotype. [*] p<0.001, Student's t test compared to the control (WT). (C) SEM of adult eyes from miR-263a mutant flies with reduced Hid activity. Left panel: miR-263a mutant with one copy of hid05014; middle panel: miR-263a mutant with one copy of the antimorphic hid allele W1; right panel: miR-263a mutant expressing a UAS-hid-RNAi transgene under control of miR-263b-Gal4. Scale bars = 50 µm. (D) Quantification of IOB numbers from flies of the genotypes shown in (C), and miR-263a mutant for comparison. Error bars represent mean ± SD for N = 20–25 flies per genotype. [*] p<0.001, Student's t test comparing to the miR-263a mutant.