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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Am J Transplant. 2009 Dec 23;10(2):262–272. doi: 10.1111/j.1600-6143.2009.02942.x

Figure 1. Dependence of Chimerism and Heart Transplant Tolerance on Residual Host CD4+ CD25+ and NK T cells.

Figure 1

A, experimental scheme: BALB/c host mice were given C57BL/6 neonatal heart transplants on day 0. ATS was injected i.p. on days 0, 2, 6, 8 and 10. TLI was given as 10 doses of 240cGy each over 14 days. On day 15, 50×106 C57BL/6 bone marrow cells were injected i.v. Some hosts were given a single dose of anti-CD25 mAb i.p. at day -7. B, heart graft survival in wild type hosts after TLI/ATS conditioning with or without bone marrow cell infusion (WT-H/T/A/B) (N=19) or (WT-H/T/A) (N=12). C, heart graft survival in CD1d-/- hosts after TLI/ATS conditioning and bone marrow cell infusion (CD1d-/- -H/T/A/B) (N=10) or in wild type hosts after anti-CD25 antibody injection, TLI/ATS, and bone marrow cell infusion (WT-H/T/A/B; CD25 dep) (N=12). D, peripheral blood chimerism in the latter hosts. The fraction of chimeric hosts in each group and the mean percentage of donor type cells among chimeric hosts are shown.