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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Am J Transplant. 2009 Dec 23;10(2):262–272. doi: 10.1111/j.1600-6143.2009.02942.x

Figure 2. Heart Transplant Recipients Conditioned with TLI and ATS Have an Increased Proportion of CD4+ CD25+ Cells Among All CD4+ T Cells After Conditioning.

Figure 2

A, representative FACS patterns of CD25 versus CD4 among CD4+ gated splenocytes in untreated wild type BALB/c mice (WT-UT), TLI/ATS conditioned wild type mice given heart transplants (WT-H/T/A), CD25 depleted and conditioned wild type hosts (WT-H/T/A) (CD25 dep) and conditioned CD1d-/- BALB/c hosts (CD1d-/- -H/T/A) 24 hours after the completion of conditioning. B, Mean percentages (± SE) of CD4+CD25+ cells among total CD4+ cells in groups shown in panel A (N=5). C, staining of Foxp3 versus CD4 in groups shown in panel A. D, mean percentages of CD4+CD25+ cells among CD4+ cells in groups in panel C (N=5). E, staining of CD25 versus Foxp3 among Foxp3+ gated cells in groups shown in panel A. F, mean percentages of CD4+CD25+Foxp3+ cells among CD4+ cells in groups shown in panel E (N=5). G, mean percentages of CD4+CD25-Foxp3+ cells among CD4+ cells in groups shown in panel E.