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Immunostaining of COPD fibroblasts. Isolated fibroblasts were immunostained to verify their mesenchymal origin. Cell nuclei are visualized by DAPI staining, shown in blue. A--D (upper panel): representative micrographs of central fibroblasts. E--H (lower panel) show distal fibroblasts. Antibodies to α-SMA were used in A and E, antibodies to prolyl 4-hydroxylase in B and F, antibodies to vimentin in C and G, and antibodies to sm22 in D and H. Scale bars represent 50 μm
