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. 2010 May 17;11(1):59. doi: 10.1186/1465-9921-11-59

Figure 3.

Figure 3

Immunohistochemical staining for claudins 1, 3, 4, 5 and 7 in the saccular period i.e. weeks 28-36. 3A. Alveolar epithelium is negative for claudin-1. Scale bar = 80 μm. 3B-C. Type II pneumocytes of developing alveoli are positive for claudin-3 (arrows). Scale bar = 80 μm in the Fig 1B, scale bar = 25 μm in the Fig 1C. 3D. Type II pneumocytes lining alveoli are positive for claudin-4 (arrow). Scale bar = 80 μm. 3E. Endothelial cells of capillaries (arrow) and an artery (arrowhead), and also epithelium of a bronchiole (short arrow) are positive for claudin-5. Scale bar = 80 μm. 3F. Strong immunoreactivity for claudin-5 is seen in endothelial cells of an artery (arrow). Scale bar = 25 μm. 3G. Type II pneumocytes of alveoli (arrow) and epithelial cells of a bronchiole (short arrow) are expressing claudin-7. Scale bar = 80 μm. 3 H: Negative control in which the primary antibody has been substituted with non-immune rabbit mouse serum. Scale bar = 80 μm.