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. Author manuscript; available in PMC: 2011 Jun 11.
Published in final edited form as: Mol Cell. 2010 Jun 11;38(5):700–711. doi: 10.1016/j.molcel.2010.05.020

Figure 2.

Figure 2

TIP60 is destabilized by HPV18 E6 in proteasome dependent manner. (A) 293T cells were transfected with indicated plasmids and cells were harvested 48 hrs after transfection. Immunoblot with indicated antibodies are shown. 0.1, 0.25, 0.5 and 1.0 μg of C18E6 plasmid was transfected. A plasmid expressing GST was co-transfected to show that the degradation was specific to TIP60. (B) TIP60 is degraded by HPV16 E6 but not HPV16 E7. Rest as in (A). (C) Inhibition of proteasome restores TIP60 in HPV18 E6 transfected cells. 293T were transfected with indicated plasmids and MG132 was added for 4 hrs before cells were harvested. Lysates were probed with indicated antibodies. (D) TIP60 and p53 are decreased in primary human keratinocytes containing HPV18 genomic DNA. Addition of MG132 restores the levels of TIP60 and p53. Lysates from primary human keratinocytes (PHK) with or without HPV18 genome were resolved and immunoblotted with indicated antibodies.