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. Author manuscript; available in PMC: 2011 Jun 11.
Published in final edited form as: Mol Cell. 2010 Jun 11;38(5):675–688. doi: 10.1016/j.molcel.2010.03.019

Figure 7. Loss of short RNAs from polycomb target genes activated in neuronal cells.

Figure 7

A. Northern blotting for short RNAs transcribed from genes targeted by polycomb in CD4+ T-cells in RNA from PBMC (P) and from differentiated SH-SY5Y neuronal cells (N). Genes active in neuronal cells are labeled.

B. Quantitative PCR showing the expression (mean and SD, n=3) of Hes5 (grey) and Pcdh8 (white) mRNA relative to actin during the step-wise differentiation to motor neurons (PMN). EB, embryoid bodies (day 2); EB+RA, EB treated with retinoic acid (day 2, 8 hours later); NPC, neuronal precursor cells (day 3), PMN, precursor motor neuron (day 4); MN, motor neuron (day 7).

C. Northern blotting for Hes5 and Pcdh8 short RNAs and 5S rRNA in ES cells during the step-wise 4-day differentiation to PMN.

D. Our data support a model in which short RNAs transcribed from the 5′ end of polycomb target genes interact with PRC2, stabilizing its interaction with chromatin and allowing H3K27 methylation and the repression of mRNA transcription in cis.