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PCR products subjected to agarose gel electrophoresis. Lane 1: a positive control with amplified DNA from purified P. jirovecii from lung tissue. Lanes 2, 3, 5 and 6 are negative samples amplifying the internal process control. Lane 4: Sample from patient. P. jirovecii reaction is positive as both the internal process control and P. jirovecii DNA of 346 base pairs are amplified.
