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. 2010 Jun 15;5(6):e11102. doi: 10.1371/journal.pone.0011102

Figure 6. Becn1 overexpression reduces APP immunoreactivity.

Figure 6

A. CHO/hAPP cells were transduced with either a GFP LV (GFP control) or a mBecn1 LV (Becn1 o.e.). Epifluorescence microscopy was performed after staining with Becn1 and APP CT15 antibodies (Scale bar represents 25 µm). GFP LV transduced cells show very faint Becn1 immunoreactivity, while Becn1 LV transduced cells exhibit a range of Becn1 signal intensity. No GFP signal is present in the Becn1 LV cells. A random selection of cells (N = 214) was picked from the GFP LV cells and the Becn1 LV cells. The Becn1 LV cells were randomly selected in both, the APP (yellow outline) and the Becn1 (red outline) channel. B. Relative immunofluorescence of the selected cells (AU). They can be divided in low, medium, and high Becn1 expressing cells. C. Quantification of the relative APP immunofluorescence in the three cohorts. Medium Becn1 overexpression leads to a significant reduction in APP levels. Medians were compared by Man-Whitney U test. * p<0.05, ** p<0.01, *** p<0.001