Figure 6. The truncated peptide sPB1-F2_pr8 ^50–87 generates an elevated membrane conductance.

A: Addition of sPB1-F2_pr8 ^50–87 protein to the trans side of a planar lipid bilayer evokes large currents across the membrane. On top of many unresolved current fluctuations typical channel like opening and closing events are resolvable. In the example recorded at +80 mV three distinct current levels (indicated y different symbols) can be identified. B: a plot of similar resolvable current levels as a function of voltage reveals three linear I/V relations with 330, 550 and 750 pS. The symbols in A cross reference with symbols in B. Measurements were done in symmetric 500 mM KCl with 200 nM peptide concentration.