. 2009 Dec 18;66(3):547–558. doi: 10.1007/s00280-009-1195-2

Table 3.

Gene expression of FPGS and pemetrexed targets

Cell line	FPGS^a	TS	DHFR	GARFT	Ratio^b
HT29	8.41 ± 0.34	7.97 ± 0.22	16.55 ± 0.83	5.28 ± 0.19	0.01
WiDr	11.32 ± 0.44	6.12 ± 0.20	6.93 ± 0.27	4.00 ± 0.15	0.07
SW620	29.31 ± 1.05	6.32 ± 0.24	7.07 ± 0.30	8.18 ± 0.42	0.08
LS174T	3.61 ± 0.11	3.98 ± 0.16	5.32 ± 0.21	1.93 ± 0.09	0.09

^aMean values calculated from standard curves, normalized with respect to expression values of the housekeeping gene GAPDH

^bRatio calculated with the formula FPGS/(TS × DHFR × GARFT); SD < 10% in all cases