| Rabbit anti-protein gene product 9.5 (PGP9.5); General marker of peripheral nerve fibres [26]
|
1∶500 |
Affinity Research |
PG9500 |
| Rabbit anti-vesicular glutamate transporter type 1 (VGLUT1); Present in annulospiral endings [22], [23]
|
1∶2000 |
Synaptic Systems |
135 302 |
| Sheep anti-calcitonin gene-related peptide (CGRP); Present in TG masseter afferents [81]
|
1∶500 |
Abcam |
ab22560 |
| Guinea pig anti-substance P (SP); Present in TG masseter afferents [81]
|
1∶1000 |
Chemicon |
AB5892 |
| Guinea pig anti-transient receptor potential vanilloid type 1 (TRPV1); Present in TG neurons [94]
|
1∶500 |
Neuromics |
GP14100 |
| Control peptide for TRPV1 |
20 µg/ml |
Neuromics |
P14100 |
| Guinea pig anti-ATP-gated purinergic receptor subunit 3 (P2X3); Present in TG masseter afferents and affected by masseter inflammation [83]
|
1∶500 |
Neuromics |
GP10108 |
| Control peptide for P2X3 |
10−5 M |
Neuromics |
P10108 |
| Rabbit anti-AMPA receptor subunit 1 (GluR1); Present in TG neurons and increased by masseter inflammation [95]
|
1∶2500 |
Immunostar Inc. |
24439 |
| Rabbit anti-NMDA receptor subunit R2B (NMDAR2B); Present in TG masseter afferents [96]; increased by masseter inflammation [95]
|
1∶100 |
Abcam |
ab109 |
| Rabbit anti-metabotropic glutamate receptor type 5 (mGluR5); Present in TG neurons; increased by masseter inflammation [95]
|
1∶500 |
Chemicon |
AB5675 |
| Control peptide for mGluR5 |
10 µg/ml |
Chemicon |
AG374 |
| Chicken anti-tyrosine hydroxylase (TH); Marker of catecholaminergic fibres [97], [98]
|
1∶100 |
Chemicon |
AB9702 |
|
Lectin
|
|
|
|
| Isolectin B4 from Griffonia simplicifolia conjugated to Alexa Fluor 488; Present in TG masseter afferents [81]
|
20 µg/ml |
Invitrogen |
I21411 |
|
Secondary antibodies
|
|
|
|
| Goat anti-rabbit-Alexa Fluor 594 |
1∶200 |
Invitrogen |
A11012 |
| Donkey anti-sheep-Alexa Fluor 488 |
1∶200 |
Invitrogen |
A11015 |
| Goat anti-guinea pig-Alexa Fluor 488 |
1∶200 |
Invitrogen |
A11073 |
| Donkey anti-chicken-FITC |
1∶200 |
Jackson |
703-095-155 |
