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. 2010 Jun 15;5(6):e11134. doi: 10.1371/journal.pone.0011134

Figure 1. Early Wnt/β-catenin signaling enhances cardiac differentiation.

Figure 1

(A) Quantitative RT-PCR on day 14 of differentiation shows induction of β-MHC, cTnT and Nkx2.5 expression by activin A/BMP4 is attenuated with Dkk1. Data are presented as mean fold expression normalized to HPRT relative to control cells. (B) Flow cytometry analysis for sarcomeric myosin heavy chain (sMHC) on day 21 of differentiation shows enhanced cardiac differentiation with addition of exogenous Wnt3a and decreased cardiac differentiation with Dkk1. (C) Late addition of Dkk1 enhanced day 14 β-MHC expression relative to samples treated with activin A/BMP4 only. Data are presented as mean fold expression normalized to HPRT relative to control cells. *p<0.05 relative to control, **p<0.01 relative to control, †p<0.05 relative to activin A/BMP4, ‡p<0.01 relative to activin A/BMP4.