Strains |
E. coli BW25113 |
rrnB
T14 DlacZWJ16 hsdR514 DaraBAD
AH33 DrhaBAD
LD78
|
(Datsenko and Wanner 2000) |
E. coli XL-1 Blue |
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacI
q
Z∆M15 Tn10 (TetR)] |
Stratagene |
C. glutamicum ATCC 13032 |
Wild type (WT) |
American Type Culture Collection |
∆aceE
|
∆aceE (Cg2466) |
This study |
∆ilvE
|
∆ilvE (Cg2418) |
This study |
∆ppc
|
∆ppc (Cg1787) |
This study |
∆pyc
|
∆pyc (Cg0791) |
This study |
∆aceE∆ldh
|
∆aceE∆ldh (Cg3219) |
This study |
∆aceE∆ldh∆pgi
|
∆aceE∆ldh∆pgi (Cg0973) |
This study |
Plasmids |
pK19mobsacB |
KanR; mobilizable (oriT, oriV); sacB; lacZα |
(Schäfer et al. 1994) |
pZA31 |
P15A ori; CmR; PLtetO1::luc
|
(Lutz and Bujard 1997) |
pSA55 |
ColE1 ori; AmpR; PLlacO1::kivd-ADH2
|
(Atsumi et al. 2008b) |
pSA69 |
p15A ori; KanR; PLlacO1::alsS-ilvC-ilvD
|
(Atsumi et al. 2008b) |
pEP2 |
NG2 ori; KanR
|
(Messerotti et al. 1990) |
pKS122 |
NG2 ori; KanR; MCS |
This study |
pKS133 |
NG2 ori; KanR; cat
|
This study |
pKS140 |
NG2 ori; KanR; Peftu::cat
|
This study |
pKS149 |
NG2 ori; KanR; Peftu::alsS
|
This study |
pKS152 |
NG2 ori; KanR; Peftu::alsS-ilvCD
|
This study |
pKS154 |
NG2 ori; KanR; Peftu::alsS-ilvCD-kivd
|
This study |
pKS160 |
NG2 ori; KanR; Peftu:: |
This study |
pKS167 |
NG2 ori; KanR; Peftu::alsS-ilvCD-kivd-adhA
|
This study |
p∆aceE
|
As pK19mobsacB but with aceE fragment |
This study |
p∆ilvE
|
As pK19mobsacB but with ilvE fragment |
This study |
p∆ldh
|
As pK19mobsacB but with ldh fragment |
This study |
p∆pgi
|
As pK19mobsacB but with pgi fragment |
This study |
p∆ppc
|
As pK19mobsacB but with ppc fragment |
This study |
p∆pyc
|
As pK19mobsacB but with pyc fragment |
This study |