Table 1.
Strains and plasmids used in this study
| Name | Relevant genotype | Reference |
|---|---|---|
| Strains | ||
| E. coli BW25113 | rrnB T14 DlacZWJ16 hsdR514 DaraBAD AH33 DrhaBAD LD78 | (Datsenko and Wanner 2000) |
| E. coli XL-1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacI q Z∆M15 Tn10 (TetR)] | Stratagene |
| C. glutamicum ATCC 13032 | Wild type (WT) | American Type Culture Collection |
| ∆aceE | ∆aceE (Cg2466) | This study |
| ∆ilvE | ∆ilvE (Cg2418) | This study |
| ∆ppc | ∆ppc (Cg1787) | This study |
| ∆pyc | ∆pyc (Cg0791) | This study |
| ∆aceE∆ldh | ∆aceE∆ldh (Cg3219) | This study |
| ∆aceE∆ldh∆pgi | ∆aceE∆ldh∆pgi (Cg0973) | This study |
| Plasmids | ||
| pK19mobsacB | KanR; mobilizable (oriT, oriV); sacB; lacZα | (Schäfer et al. 1994) |
| pZA31 | P15A ori; CmR; PLtetO1::luc | (Lutz and Bujard 1997) |
| pSA55 | ColE1 ori; AmpR; PLlacO1::kivd-ADH2 | (Atsumi et al. 2008b) |
| pSA69 | p15A ori; KanR; PLlacO1::alsS-ilvC-ilvD | (Atsumi et al. 2008b) |
| pEP2 | NG2 ori; KanR | (Messerotti et al. 1990) |
| pKS122 | NG2 ori; KanR; MCS | This study |
| pKS133 | NG2 ori; KanR; cat | This study |
| pKS140 | NG2 ori; KanR; Peftu::cat | This study |
| pKS149 | NG2 ori; KanR; Peftu::alsS | This study |
| pKS152 | NG2 ori; KanR; Peftu::alsS-ilvCD | This study |
| pKS154 | NG2 ori; KanR; Peftu::alsS-ilvCD-kivd | This study |
| pKS160 | NG2 ori; KanR; Peftu:: | This study |
| pKS167 | NG2 ori; KanR; Peftu::alsS-ilvCD-kivd-adhA | This study |
| p∆aceE | As pK19mobsacB but with aceE fragment | This study |
| p∆ilvE | As pK19mobsacB but with ilvE fragment | This study |
| p∆ldh | As pK19mobsacB but with ldh fragment | This study |
| p∆pgi | As pK19mobsacB but with pgi fragment | This study |
| p∆ppc | As pK19mobsacB but with ppc fragment | This study |
| p∆pyc | As pK19mobsacB but with pyc fragment | This study |