Skip to main content
. 2010 Jun 15;5(6):e11133. doi: 10.1371/journal.pone.0011133

Figure 4. Functional characterization of mammalian TAAR3, TAAR4 and TAAR5 orthologs using a CRE-SEAP reporter gene assay.

Figure 4

HEK293 cells were transiently co-transfected with CRE-SEAP reporter plasmid (Clontech) and respective receptor orthologs and tested for agonist-induced SEAP-activity. The basal value of non-stimulated mock-transfected HEK293 determined was 193,208±21,052 cpm/well. Data are given as mean±SEM of 2 to 5 independent experiments each performed in triplicates. Concentration-response curves of agonists were determined using GraphPad Prism.