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Alignment of amino acid sequences of IbCAD1 and other plant CADs. Conserved important regions identified previously are marked as follows: inverted filled triangle denotes catalytic zinc ion coordinating residue, filled circle denotes structural Zn ion coordinating residue, star denotes key residues for substrate specificity. Locations of the Zn1, Zn2, and NADPH binding domains are shown in boxes. The alignment was performed with the ClustalX and GENEDOC software programs
