Fig. 5.

GUS activity and histochemical localization of the IbCAD1 promoter at different developmental stages of transgenic tobacco plants. A Transgenic tobacco seedlings were germinated on MS medium and collected at 5, 8, and 12 days after germination (DAG). GUS activity was measured in the cotyledons and roots, respectively. The data represent the average value ± SD from independent experiments (n = 40). Fully developed leaves, petioles, upper stems, and root materials were collected from 4- to 8-week-old mature plants grown in soil and analyzed for GUS activity. The data represent the average value ± SD from independent experiments (n = 6). B (a) Seedling with fully developed cotyledon at 5 DAG; (b) seedling with fully matured cotyledons at 8 DAG; (c) seedling with expanding secondary leaves and initiating lateral roots at 12 DAG; (d) root tip of seedling at 12 DAG; (e) initiation regions of lateral roots at 12 DAG; (f, g) root of 4-week-old plant; (h) root of 8-week-old plant; (i) transverse section of stem; (j) longitudinal section of stem; (k) leaf of 4-week-old plant; (l) stigma; (m) anther; (n) filament; (o) pollen grains