. 2010 Apr 20;87(3):1167–1176. doi: 10.1007/s00253-010-2595-2

Table 2.

Primers used for PCR amplification

Target gene		Primer	Sequence (5′–3′)	PCR program	Reference
16S rRNA	Bacterial	EBU8F	AGAGTTTGATCMTGGCTCAG	(94°C, 60 s; 55°C, 45 s; 72°C, 60 s) × 30	Heuer et al. 1997
	Bacterial	UNIV1392R	ACGGGCGGTGTGTRC	(94°C, 60 s; 55°C, 45 s; 72°C, 60 s) × 30	Ferris et al. 1996
	Archaeal	ARC23F	TGCAGAYCTGGTYGATYCTGCC	(94°C, 45 s; 56°C, 45 s; 72°C, 60 s) × 35	Burggraf et al. 1991
	Archaeal	ARC934R	GTGCTCCCCGCCAATTCCT	(94°C, 45 s; 56°C, 45 s; 72°C, 60 s) × 35	Giovannoni et al. 1998
amoA	AOA	cren amo_F	ATGGTCTGGCTAAGACGMTGTA	(94°C, 45 s; 55°C, 45 s; 72°C, 45 s) × 35	Hallam et al. 2006
	AOA	amoAR^a	GCGGCCATCCATCTGTATGT	(94°C, 45 s; 55°C, 45 s; 72°C, 45 s) × 35	Francis et al. 2005
	AOB	amoA-1F	GGGGTTTCTACTGGTGGT	(94°C, 60 s; 55°C, 45 s; 72°C, 60 s) × 40	Rotthauwe et al. 1997
	AOB	amoA-2R^a	CCCCTCKGSAAAGCCTTCTTC	(94°C, 60 s; 55°C, 45 s; 72°C, 60 s) × 40	Rotthauwe et al. 1997

^aPrimers used for T-RFLP analysis were labeled by Hex