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. 2010 Apr 1;121(2):393–402. doi: 10.1007/s00122-010-1318-5

Table 2.

Newly developed microsatellite markers derived from the BAC sequence of clone 71F18 (GenBank entry DQ323045)

Marker Forward primer Tm
For
Reverse primer Tm
Rev
BAC position Motif Expected allele size TD Parental polymor.a
BMarc1 GCTAGCGCTTCTCTTATTCA 52.2 AAGAAAAACCCTACCTTTGG 50.3 14078–14240 (CT)5 162 55–45 M
BMarc2 AGGGAAGGAAGAGAAAAAGA 50.7 AACCTTCCTCTACCTCAACC 53.7 15599–15785 (GCT)5 186 56–46 M
BMarc3 TGAGAGGAGTCAAAAACCAT 51.3 GAAGACCTACACGCAACTTT 52.8 25473–25757 (AT)5 284 56–46 D
BMarc4 TTATCACCATCACCCAAGTT 51.4 CCTTCGTGTTTGTTTGTGTT 51.8 39303–39455 (ATAC)3 152 56–46 Db
BMarc5 TCGTGTTTATGTGGTATCAG 49.6 GCCTAATGCAAGTTATCCAA 50.5 42458–42737 (TTA)9 279 55–45 Db
BMarc6 CCGATCAATACATAGTATGCTG 50.7 CAACCCACCAATTCTACTCT 51.9 45308–45486 (ATGA)3 178 56–46 D
BMarc7 GTCTTCTGCAGGTACCATCT 54.0 CCAAAGAACTATTGGGATCA 49.6 49306–49457 (CCGA)3 151 55–45 C
BMarc8 CGTGAGACGAGTCAATAACC 52.9 GAAGACCTACACGCAACTTT 52.8 69383–69675 (AT)7 292 58–48 D
BMarc9 TTCGAGGTGACGTAAAGATT 51.2 TCAACAAGGATGGAGAAGAT 50.8 69774–69963 (AAT)7 189 56–46 D
BMarc10 CGAGCATCGAATACCTTTAC 50.8 GGATCACTCTTCCTCTTTCTT 51.6 64136–64311 (CGAT)3 175 56–46 Db
BMarc11 TAAATCGCCACAGAAGGA 50.7 GGGGGTTTTCTCTTGTTAAG 50.9 77734–77927 (GT)5 193 56–46 NA
BMarc12 GAAAACGATGCACACAATC 49.9 TATGTTGTACCATCGGTTGA 51.0 75269–75468 (ATAA)3 199 55–45 Db
BMarc13 TGTAGGTACCATTCGAGTCC 53.4 CACAAATTGTTCTCCATCG 49.1 84872–85024 (GACC)3 152 54–44 D
BMarc14 GGAAGACACATGTGAAGAGG 52.9 CTTCCTCTCACTTTCCACTG 52.6 88292–88454 (GCAT)5 162 58–48 Db
BMarc15 AAGAGGGCTAGGGAGAGTAA 54.2 GCCATCGTTGCTTAATTCT 50.7 91408–91578 (CT)6 170 56–46 Db
BMarc16 TCATGCATTAACCATCACTC 50.2 GCCATCGTTGCTTAATTCT 50.7 97416–97560 (CT)7 144 55–45 Cb
BMarc17 CTTCTGAGCTGAAGTAAAACG 51.3 AGTGAGACAGAAAGGCAAAT 51.8 113764–114010 (AATT)3 246 56–46 Cb
BMarc18 CTAGCCAGACCTCAGACATC 54.3 GCATGAATCTTCTCGTGACT 52.4 125636–125796 (GA)5 160 57–47 M
BMarc19 TATGTCCTACGAGGGTTGAT 52.4 CCCGTTGAAAAGATCAAGT 50.3 136669–136833 (AT)12 164 55–45 NA
BMarc20 AGCTGAAGAACGAGGACAT 53.5 GAGAAGCACTTCATCGTCAT 52.4 139381–139527 (CG)5 146 57–47 M
BMarc21 AGGACACATGGACATGGATA 53.0 AAGAAGGGGATGAAATGTCT 51.1 141812–142037 (AT)11 225 56–46 Cb
BMarc22 GAGTTTTGGGGAGACATTG 50.9 ATTTGAGAGCGAAAGATCAC 50.4 152436–152580 (GGC)6 144 55–45 Cb

TD touchdown profile with upper and lower annealing temperatures in °C, C co-dominant polymorphic, D dominant polymorphic, M monomorphic, NA no amplification

aPolymorphism parental survey

bIndicates if marker had clear amplification product in the population and was used for mapping