Table 2.

Newly developed microsatellite markers derived from the BAC sequence of clone 71F18 (GenBank entry DQ323045)

Marker	Forward primer	Tm For	Reverse primer	Tm Rev	BAC position	Motif	Expected allele size	TD	Parental polymor.a
BMarc1	GCTAGCGCTTCTCTTATTCA	52.2	AAGAAAAACCCTACCTTTGG	50.3	14078–14240	(CT)5	162	55–45	M
BMarc2	AGGGAAGGAAGAGAAAAAGA	50.7	AACCTTCCTCTACCTCAACC	53.7	15599–15785	(GCT)5	186	56–46	M
BMarc3	TGAGAGGAGTCAAAAACCAT	51.3	GAAGACCTACACGCAACTTT	52.8	25473–25757	(AT)5	284	56–46	D
BMarc4	TTATCACCATCACCCAAGTT	51.4	CCTTCGTGTTTGTTTGTGTT	51.8	39303–39455	(ATAC)3	152	56–46	Db
BMarc5	TCGTGTTTATGTGGTATCAG	49.6	GCCTAATGCAAGTTATCCAA	50.5	42458–42737	(TTA)9	279	55–45	Db
BMarc6	CCGATCAATACATAGTATGCTG	50.7	CAACCCACCAATTCTACTCT	51.9	45308–45486	(ATGA)3	178	56–46	D
BMarc7	GTCTTCTGCAGGTACCATCT	54.0	CCAAAGAACTATTGGGATCA	49.6	49306–49457	(CCGA)3	151	55–45	C
BMarc8	CGTGAGACGAGTCAATAACC	52.9	GAAGACCTACACGCAACTTT	52.8	69383–69675	(AT)7	292	58–48	D
BMarc9	TTCGAGGTGACGTAAAGATT	51.2	TCAACAAGGATGGAGAAGAT	50.8	69774–69963	(AAT)7	189	56–46	D
BMarc10	CGAGCATCGAATACCTTTAC	50.8	GGATCACTCTTCCTCTTTCTT	51.6	64136–64311	(CGAT)3	175	56–46	Db
BMarc11	TAAATCGCCACAGAAGGA	50.7	GGGGGTTTTCTCTTGTTAAG	50.9	77734–77927	(GT)5	193	56–46	NA
BMarc12	GAAAACGATGCACACAATC	49.9	TATGTTGTACCATCGGTTGA	51.0	75269–75468	(ATAA)3	199	55–45	Db
BMarc13	TGTAGGTACCATTCGAGTCC	53.4	CACAAATTGTTCTCCATCG	49.1	84872–85024	(GACC)3	152	54–44	D
BMarc14	GGAAGACACATGTGAAGAGG	52.9	CTTCCTCTCACTTTCCACTG	52.6	88292–88454	(GCAT)5	162	58–48	Db
BMarc15	AAGAGGGCTAGGGAGAGTAA	54.2	GCCATCGTTGCTTAATTCT	50.7	91408–91578	(CT)6	170	56–46	Db
BMarc16	TCATGCATTAACCATCACTC	50.2	GCCATCGTTGCTTAATTCT	50.7	97416–97560	(CT)7	144	55–45	Cb
BMarc17	CTTCTGAGCTGAAGTAAAACG	51.3	AGTGAGACAGAAAGGCAAAT	51.8	113764–114010	(AATT)3	246	56–46	Cb
BMarc18	CTAGCCAGACCTCAGACATC	54.3	GCATGAATCTTCTCGTGACT	52.4	125636–125796	(GA)5	160	57–47	M
BMarc19	TATGTCCTACGAGGGTTGAT	52.4	CCCGTTGAAAAGATCAAGT	50.3	136669–136833	(AT)12	164	55–45	NA
BMarc20	AGCTGAAGAACGAGGACAT	53.5	GAGAAGCACTTCATCGTCAT	52.4	139381–139527	(CG)5	146	57–47	M
BMarc21	AGGACACATGGACATGGATA	53.0	AAGAAGGGGATGAAATGTCT	51.1	141812–142037	(AT)11	225	56–46	Cb
BMarc22	GAGTTTTGGGGAGACATTG	50.9	ATTTGAGAGCGAAAGATCAC	50.4	152436–152580	(GGC)6	144	55–45	Cb

TD touchdown profile with upper and lower annealing temperatures in °C, C co-dominant polymorphic, D dominant polymorphic, M monomorphic, NA no amplification

^aPolymorphism parental survey

^bIndicates if marker had clear amplification product in the population and was used for mapping