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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Mar 6;1801(8):917–923. doi: 10.1016/j.bbalip.2010.02.012

Figure 4.

Figure 4

24S-hydroxycholesterol modulates the expression of RORα target genes. 24S-hydroxycholesterol (10 μM) suppresses the expression of BMAL1 (A) and REV-ERBα (B) mRNA in a RORα dependent manner in HepG2 cells. (C) Expression of RORα following treatment of cells with siRNA targeting RORα. Control cells received scrambled siRNA. (D) ChIP-reChIP assay illustrating the ability of 24S-OHC to decrease SRC-2 recruitment to RORα occupying the BMAL1 promoter. The graph below the gel indicates the average band intensity from three experiments. IgG is used as a negative control and α-RNA pol II antibody is used as a positive control. * indicates p<0.05.