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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: Cancer Growth Metastasis. 2010 Jan 20;2009(2):45–55.

Figure 2.

Figure 2

BITC and TRAIL-induced apoptosis in BxPC3, MiaPaCa2, and Panc-1 cells. Analysis of apoptotic induction in BxPC3, MiaPaCa2, and Panc-1 cells using the cell death ELISA assay. BxPC3, MiaPaCa2, and Panc-1 cells were plated at 1 × 105 cells/ml. Cells were treated with vehicle, 5 µM BITC 24 hrs, and 10 ng/ml TRAIL for 6 hrs or both BITC and TRAIL. The pan-caspase inhibitor, Q-VD-OPh (QVD) was added at 5 µM for 30 minutes prior to treatment where indicated. Cell death ELISA results are represented by fold change in apoptosis compared to the vehicle. Error bars represent standard deviation. *Denotes significance to p ≤ 0.05 when compared with control. #Denotes significance to p ≤ 0.05 when comparison was made between TRAIL alone and BITC + TRAIL.