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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: Cancer Growth Metastasis. 2010 Jan 20;2009(2):45–55.

Figure 4.

Figure 4

Analysis of the intrinsic apoptotic pathway in human pancreatic cancer cells. Activation of caspase 9 was evaluated in BxPC3, MiaPaCa2 and Panc-1 cell lines upon treatment with vehicle (V), 5 µM BITC for 24 hrs (B), 10 ng/ml TRAIL (T) for 6 hrs or both 5 µM BITC and 10 ng/ml TRAIL (BT). Total treatment time was 24 hours. Whole cell lysates were collected, separated, and analyzed by Western blotting described in the Methods and Materials. The blots were probed with antibodies to initiator caspase 9 A), caspase 3 B), or PARP C). Arrows indicated procaspase 9 and the cleaved/active fragment. Asterisk (*) indicates an unrelated, crossreactive band. Jurkat human T cells treated with vehicle (−), actinomycin (+) (A, B), or anti-fas (+) (C) were used as negative and positive controls, respectively.