Figure 1. Development of MIIG transgenic mice.

A. Diagram of the MIIG transgenic construct; a CD68 promoter/intron fragment was combined with a myc-tagged, dominant negative, mutant IFN-γ receptor 1 chain, which has a truncation of the cytoplasmic signaling portion. B. This promoter/gene construct was tested by transfection into L929 cells. Upregulation of MHC class I (K^k) in response to increasing amounts of IFN-γ was measured in transduced and non-transduced cells. C. Flow cytometric analysis of murine spleen cells reveals that CD68 protein is highly expressed in macrophages and to a lesser degree in CD11c^high (dendritic) cells.