Figure 5. Macrophages from MIIG mice are unable to kill intracellular parasites or produce nitric oxide in response to IFN-γ.
A. Bone marrow derived macrophages were infected with T. cruzi (Trypo) and treated as indicated. NO production and clearance of T. cruzi infection was measured at 48 hours. B. Peritoneal macrophages were infected with L. major and cultured as indicated. NO production was measured at 72 hours. Images (600X) of L. major infected macrophages +/− IFN-γ/LPS treatment at 96 hours, with numerous visible parasites. *=p<0.01 comparing MIIG and WT macrophages treated in the same fashion. n=3 animals, with all studies repeated at least twice.