Table I.
Kinetochore Fic and protein number per kinetochore and kMT for GFP fusion cell lines
| GFP fusion cell lines | Fic | Ratio to yeast Ndc80 | Protein No./kinetochore | Protein No./kMT |
| Budding yeast Ndc80 | 3,390 ± 373 | 1.00 ± 0.11 | NA | NA |
| CENP-C | 1,192 ± 312 | 0.35 ± 0.09 | 39 ± 10 | 9.2 ± 2.4 |
| CENP-I | 757 ± 113 | 0.22 ± 0.03 | 25 ± 4 | 5.8 ± 0.9 |
| CENP-T | 851 ± 119 | 0.25 ± 0.04 | 28 ± 4 | 6.5 ± 0.9 |
| CENP-H CMV | 879 ± 147 | 0.26 ± 0.04 | 29 ± 5 | 6.8 ± 1.1 |
| CENP-H (KI) | 808 ± 121 | 0.24 ± 0.04 | 27 ± 4 | 6.2 ± 0.9 |
| Mis12 | 1,172 ± 271 | 0.35 ± 0.08 | 39 ± 9 | 9.0 ± 2.1 |
| KNL-1 | 932 ± 170 | 0.28 ± 0.05 | 31 ± 6 | 7.2 ± 1.3 |
| Ndc80 (Hec1) | 1,000 ± 178 | 0.29 ± 0.05 | 33 ± 6 | 7.7 ± 1.4 |
| Nuf2 | 1,128 ± 251 | 0.33 ± 0.07 | 37 ± 8 | 8.7 ± 1.9 |
NA, not applicable. Mean values for Fic were measured from kinetochores for each GFP fusion cell line, and calculations of protein copy number per kinetochore and kMT were obtained by the ratio fluorescence method. Ratio values to yeast Ndc80-GFP were obtained by dividing the mean Fic by the value for yeast Ndc80-GFP. The protein number per kinetochore in DT40 cells was obtained by multiplying the ratio to yeast Ndc80 by the number of Ndc80-GFPs in an anaphase kinetochore cluster (112; Joglekar et al., 2006). The protein number per kMT was obtained by dividing the ratio to yeast Ndc80 by the mean number of kMTs in metaphase DT40 cells (4.3; Ribeiro et al., 2009).