Figure 8.

The CC2 and tail domains interact directly with the KIF17 motor. (A) Schematic of GST-tagged constructs. (B) Coomassie-stained gel showing recombinant, purified GST-(1–490)-Myc motor, WT and mutant GST-CC2, and WT and mutant GST-tail proteins. (C) GST-(1–490)-Myc protein was bound to an anti-Myc column, then GST-tagged versions of WT or mutant (764–772A) CC2 domains as well as WT or mutant (1016–1019A) tail domains were added to the column. GST was used as a control. The column-bound proteins were identified by immunoblotting with an anti-GST antibody. Boxed regions indicate proteins of interest. The numbers to the left of the gel blots indicate protein size markers in kD.