Figure 2.

Polyglutamylation activates 58-kD spastin in vivo. (A) HeLa cells were cotransfected with the 58-kD isoform of spastin-EYFP (mutated S359C; Fig. S3) and an active (TTLL11) or inactive (TTLL11d) polyglutamylase. Cells were grown for 16 h before fixation and immunofluorescence staining with 12G10. CFP allowed identification of TTLL11-expressing cells. Bar, 20 µm. (B) Fraction cells expressing spastin S359C-EYFP and CFP that show MT severing as shown in A. A minimum of 100 cells was analyzed in three independent experiments. Error bars indicate SD. Significance was determined by two-tailed Student’s t test. P values are below 0.001 (**), or below 0.01 (*).